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rabbit anti-mouse cd8 polyclonal  (MyBiosource Biotechnology)

 
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    MyBiosource Biotechnology rabbit anti-mouse cd8 polyclonal
    Rabbit Anti Mouse Cd8 Polyclonal, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-mouse+cd8+polyclonal/rabbit+anti+mouse+cd8+polyclonal/pm31432168-56-43-50
    Average 90 stars, based on 1 article reviews
    rabbit anti-mouse cd8 polyclonal - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model.
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4 ̊C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti‐mouse CD4 monoclonal (cat. no. 14‐9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti‐mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti‐mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti‐mouse CD68 polyclonal (cat. no. bs‐0649R; Bioss, Inc.), rat anti‐mouse MHC class ii monoclonal (cat. no. sc-59318cat), rat anti-mouse iFn-γ monoclonal (cat. no. sc‐74104), goat anti‐mouse IL‐12 ap35 polyclonal (cat. no. sc-9350), rat anti-mouse il-12p40 monoclonal (cat. no. sc‐57258), rat anti‐mouse IL‐4 monoclonal (cat. no. sc‐73318), rabbit anti‐mouse IL‐5 polyclonal (cat. no. sc‐7887), goat anti‐mouse IL‐13 polyclonal (sc‐1776), goat anti‐mouse IL‐6 polyclonal (cat. no. sc‐1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti‐mouse TnF-α polyclonal antibody (cat. no. 3053R‐100; BioVision, inc.).

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4°C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti-mouse CD4 monoclonal (cat. no. 14-9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti-mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti-mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti-mouse CD68 polyclonal (cat. no. bs-0649R; Bioss, Inc.), rat anti-mouse MHC class II monoclonal (cat. no. sc-59318cat), rat anti-mouse IFN-γ monoclonal (cat. no. sc-74104), goat anti-mouse IL-12 Ap35 polyclonal (cat. no. sc-9350), rat anti-mouse IL-12p40 monoclonal (cat. no. sc-57258), rat anti-mouse IL-4 monoclonal (cat. no. sc-73318), rabbit anti-mouse IL-5 polyclonal (cat. no. sc-7887), goat anti-mouse IL-13 polyclonal (sc-1776), goat anti-mouse IL-6 polyclonal (cat. no. sc-1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti-mouse TNF-α polyclonal antibody (cat. no. 3053R-100; BioVision, Inc.).

    Blocking Assay:

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model.
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4 ̊C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti‐mouse CD4 monoclonal (cat. no. 14‐9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti‐mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti‐mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti‐mouse CD68 polyclonal (cat. no. bs‐0649R; Bioss, Inc.), rat anti‐mouse MHC class ii monoclonal (cat. no. sc-59318cat), rat anti-mouse iFn-γ monoclonal (cat. no. sc‐74104), goat anti‐mouse IL‐12 ap35 polyclonal (cat. no. sc-9350), rat anti-mouse il-12p40 monoclonal (cat. no. sc‐57258), rat anti‐mouse IL‐4 monoclonal (cat. no. sc‐73318), rabbit anti‐mouse IL‐5 polyclonal (cat. no. sc‐7887), goat anti‐mouse IL‐13 polyclonal (sc‐1776), goat anti‐mouse IL‐6 polyclonal (cat. no. sc‐1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti‐mouse TnF-α polyclonal antibody (cat. no. 3053R‐100; BioVision, inc.).

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4°C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti-mouse CD4 monoclonal (cat. no. 14-9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti-mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti-mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti-mouse CD68 polyclonal (cat. no. bs-0649R; Bioss, Inc.), rat anti-mouse MHC class II monoclonal (cat. no. sc-59318cat), rat anti-mouse IFN-γ monoclonal (cat. no. sc-74104), goat anti-mouse IL-12 Ap35 polyclonal (cat. no. sc-9350), rat anti-mouse IL-12p40 monoclonal (cat. no. sc-57258), rat anti-mouse IL-4 monoclonal (cat. no. sc-73318), rabbit anti-mouse IL-5 polyclonal (cat. no. sc-7887), goat anti-mouse IL-13 polyclonal (sc-1776), goat anti-mouse IL-6 polyclonal (cat. no. sc-1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti-mouse TNF-α polyclonal antibody (cat. no. 3053R-100; BioVision, Inc.).

    Immunopeptidomics:

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model.
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4 ̊C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti‐mouse CD4 monoclonal (cat. no. 14‐9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti‐mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti‐mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti‐mouse CD68 polyclonal (cat. no. bs‐0649R; Bioss, Inc.), rat anti‐mouse MHC class ii monoclonal (cat. no. sc-59318cat), rat anti-mouse iFn-γ monoclonal (cat. no. sc‐74104), goat anti‐mouse IL‐12 ap35 polyclonal (cat. no. sc-9350), rat anti-mouse il-12p40 monoclonal (cat. no. sc‐57258), rat anti‐mouse IL‐4 monoclonal (cat. no. sc‐73318), rabbit anti‐mouse IL‐5 polyclonal (cat. no. sc‐7887), goat anti‐mouse IL‐13 polyclonal (sc‐1776), goat anti‐mouse IL‐6 polyclonal (cat. no. sc‐1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti‐mouse TnF-α polyclonal antibody (cat. no. 3053R‐100; BioVision, inc.).

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4°C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti-mouse CD4 monoclonal (cat. no. 14-9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti-mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti-mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti-mouse CD68 polyclonal (cat. no. bs-0649R; Bioss, Inc.), rat anti-mouse MHC class II monoclonal (cat. no. sc-59318cat), rat anti-mouse IFN-γ monoclonal (cat. no. sc-74104), goat anti-mouse IL-12 Ap35 polyclonal (cat. no. sc-9350), rat anti-mouse IL-12p40 monoclonal (cat. no. sc-57258), rat anti-mouse IL-4 monoclonal (cat. no. sc-73318), rabbit anti-mouse IL-5 polyclonal (cat. no. sc-7887), goat anti-mouse IL-13 polyclonal (sc-1776), goat anti-mouse IL-6 polyclonal (cat. no. sc-1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti-mouse TNF-α polyclonal antibody (cat. no. 3053R-100; BioVision, Inc.).

    Binding Assay:

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model.
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4 ̊C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti‐mouse CD4 monoclonal (cat. no. 14‐9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti‐mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti‐mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti‐mouse CD68 polyclonal (cat. no. bs‐0649R; Bioss, Inc.), rat anti‐mouse MHC class ii monoclonal (cat. no. sc-59318cat), rat anti-mouse iFn-γ monoclonal (cat. no. sc‐74104), goat anti‐mouse IL‐12 ap35 polyclonal (cat. no. sc-9350), rat anti-mouse il-12p40 monoclonal (cat. no. sc‐57258), rat anti‐mouse IL‐4 monoclonal (cat. no. sc‐73318), rabbit anti‐mouse IL‐5 polyclonal (cat. no. sc‐7887), goat anti‐mouse IL‐13 polyclonal (sc‐1776), goat anti‐mouse IL‐6 polyclonal (cat. no. sc‐1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti‐mouse TnF-α polyclonal antibody (cat. no. 3053R‐100; BioVision, inc.).

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4°C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti-mouse CD4 monoclonal (cat. no. 14-9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti-mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti-mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti-mouse CD68 polyclonal (cat. no. bs-0649R; Bioss, Inc.), rat anti-mouse MHC class II monoclonal (cat. no. sc-59318cat), rat anti-mouse IFN-γ monoclonal (cat. no. sc-74104), goat anti-mouse IL-12 Ap35 polyclonal (cat. no. sc-9350), rat anti-mouse IL-12p40 monoclonal (cat. no. sc-57258), rat anti-mouse IL-4 monoclonal (cat. no. sc-73318), rabbit anti-mouse IL-5 polyclonal (cat. no. sc-7887), goat anti-mouse IL-13 polyclonal (sc-1776), goat anti-mouse IL-6 polyclonal (cat. no. sc-1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti-mouse TNF-α polyclonal antibody (cat. no. 3053R-100; BioVision, Inc.).

    Immunohistochemistry:

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model.
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4 ̊C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti‐mouse CD4 monoclonal (cat. no. 14‐9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti‐mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti‐mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti‐mouse CD68 polyclonal (cat. no. bs‐0649R; Bioss, Inc.), rat anti‐mouse MHC class ii monoclonal (cat. no. sc-59318cat), rat anti-mouse iFn-γ monoclonal (cat. no. sc‐74104), goat anti‐mouse IL‐12 ap35 polyclonal (cat. no. sc-9350), rat anti-mouse il-12p40 monoclonal (cat. no. sc‐57258), rat anti‐mouse IL‐4 monoclonal (cat. no. sc‐73318), rabbit anti‐mouse IL‐5 polyclonal (cat. no. sc‐7887), goat anti‐mouse IL‐13 polyclonal (sc‐1776), goat anti‐mouse IL‐6 polyclonal (cat. no. sc‐1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti‐mouse TnF-α polyclonal antibody (cat. no. 3053R‐100; BioVision, inc.).

    Article Title: Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2-related factors in an ovalbumin-induced asthma mouse model
    Article Snippet: The slides were incubated with normal serum to block the nonspecific bindings and then incubated for 1 h at 4°C with different primary antibodies (diluted 1:100 to 1:200), such as rat anti-mouse CD4 monoclonal (cat. no. 14-9766; eBioscience; Thermo Fisher Scientific, Inc.), rabbit anti-mouse CD8 polyclonal (cat. no. MBS551004; Mybiosource, Sand Diego, CA, USA), rabbit anti-mouse CD19 polyclonal (cat. no. 250585; Abbiotec), rabbit anti-mouse CD68 polyclonal (cat. no. bs-0649R; Bioss, Inc.), rat anti-mouse MHC class II monoclonal (cat. no. sc-59318cat), rat anti-mouse IFN-γ monoclonal (cat. no. sc-74104), goat anti-mouse IL-12 Ap35 polyclonal (cat. no. sc-9350), rat anti-mouse IL-12p40 monoclonal (cat. no. sc-57258), rat anti-mouse IL-4 monoclonal (cat. no. sc-73318), rabbit anti-mouse IL-5 polyclonal (cat. no. sc-7887), goat anti-mouse IL-13 polyclonal (sc-1776), goat anti-mouse IL-6 polyclonal (cat. no. sc-1265; all from Santa Cruz Biotechnology, Inc.), and rabbit anti-mouse TNF-α polyclonal antibody (cat. no. 3053R-100; BioVision, Inc.).



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    Image Search Results


    Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red). " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Multiparameter spectral flow cytometry analysis of immune cells of the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) Automated T-distributed stochastic neighbour embedding (t-SNE) 2D map of the flow cytometry data acquired from control and infected mice colon. (B) Upper panel; tSNE 2D map showing scaled expression of CD11b for myeloid cells, B220 for B cells and CD3 for T cells. Lower panel; tSNE 2D map showing the location of CD11b + myeloid cells, B220 + B cells and CD3 + T cells. (C) Heat maps for (left) cell surface markers expression (CD45, CD11b, B220, CD3, CD4 and CD8) and (right) groups (control, acute and chronic colons) for 22 cell clusters identified. (D) Percentage of each cluster for each group. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Arrows in (C) and (D) indicate immune cell clusters of myeloid cells (blue), B cells (green) or T cells (red).

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in <xref ref-type=Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Phenotypic analysis of T cells via multiparameter spectral flow cytometry in the colonic lamina propria during acute and chronic T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Colonic lamina propria cells were isolated from uninfected (Control), acutely infected (Acute, 30 dpi) and chronically infected (Chronic, 90 dpi) mice and analysed by flow cytometry using the gating strategy shown in Supplementary Figure 1 . (A) T cells composition by flow cytometry, (B) tSNE 2D map showing scaled expression of CD3, CD4, and CD8 cell makers (C) Heat map of T cells (CD45 + , CD3 + ) (highlighted clusters 1, 7, 9, 16, 20, 22). Arrows indicate immune cell clusters of inflammatory (purple) and regulatory (pink) double-negative (DN) T cells. (D) tSNE 2D map of DN T cells and percentage of T cells cluster for each group. Arrows indicate DN T cell clusters of inflammatory (purple) and regulatory (pink) cells. (E) Percentage of DN T cells with inflammatory/regulatory phenotypes in total immune cells (CD45 + cells). (F) Relative percentage of DN T cells with inflammatory/regulatory phenotypes in total DN T cells. Panel (D) shows auto-scaled cluster frequencies optimized for visualization within each group. For standardized quantitative comparison across groups, refer to the heat map in panel (C) . Bars in A represent mean ± SD, and individual symbols denote values from single mice (n = 15; 5 per set, 3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, ****p < 0.0001.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Flow Cytometry, Infection, Isolation, Control, Expressing, Comparison

    Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Double-negative T cells phenotypes in the colonic lamina propria of C57BL/6 mice during T. cruzi infection. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc). Mice were euthanized during the acute (30 dpi) and chronic (90 dpi) phases. Colonic lamina propria cells were isolated from uninfected (Control; blue), acutely infected (Acute; pink) and chronically infected (Chronic; green) mice. Cells were gated on single cells, live, CD45 + , CD3 + , CD4 - , CD8 - events and subsequently on (A) inflammatory immune cell markers including CCR5, CXCR3 or Granzyme B, and (B) regulatory immune cells markers including CCR4, IL10Rα or IL10. Bars represent mean ± SD, and individual symbols denote values from single mice (n = 8-12; 4 per set, 2–3 individual sets). Statistical comparisons were made by an unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001, **** p ≤ 0.0001.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Infection, Isolation, Control

    Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( <xref ref-type=Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Double negative T cells (CD4 - /CD8 - ) are associated with Trypanosoma cruzi persistence in the mouse colon during chronic Chagas disease

    doi: 10.3389/fimmu.2026.1761769

    Figure Lengend Snippet: Microscopy analysis of T. cruzi infected colons. C57BL/6 mice were infected with 10 4 T. cruzi (TcCol-Nluc-RFP). Colons were isolated from control and chronically infected mice (90 dpi), processed for microscopy and stained with CD3 (Blue), CD4 (Green) and CD8 (Red) specific antibodies ( Supplementary Table 1 , Antibody Panel 4) as described in the materials and methods. Immunofluorescence images of cross sections of colons obtained from non-infected mice (Control) and chronically infected mice (Chronic) are shown in panels (A, B) , respectively. In (A, B) , a low magnification image of the entire colon is shown in the upper left, a region of interest (ROI) 1 is shown at mid-magnification in the lower left, and a high magnification image of ROI 2 is shown in shown on the right. For ROI 2, an overlay image of the 3 channels CD3 (Blue), CD4 (Green) and CD8 (Red) is shown on the top right, and the individual channels are represented below as indicated. A representative result of 3 controls and 3 infected colons is shown in this figure. LP, Lamina Propria; solid white arrow heads, double-negative T cells (CD3 + , CD4 - , CD8 - ); open arrowheads, CD3 - , CD4 - , CD8 + cells; solid pink arrowheads, CD3 - , CD4 + , CD8 - cells; solid yellow arrowheads, CD3 - , CD4 + , CD8 + cells.

    Article Snippet: Antibodies were polyclonal goat IgG anti-mouse CD4 antibody (R&D Systems, MN), polyclonal rabbit IgG anti-mouse CD8 antibody (Novus Biologicals, CO), monoclonal Rat IgG2b Alexa Fluor ® 647 anti-mouse CD3 Antibody (BioLegend, CA), Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Thermo Fisher Scientific, CA), Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (Thermo Fisher Scientific, CA) ( ).

    Techniques: Microscopy, Infection, Isolation, Control, Staining, Immunofluorescence